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human normal embryonic lung fibroblast cell line mrc5  (ATCC)


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    ATCC human normal embryonic lung fibroblast cell line mrc5
    (A) Quantification of mean number of SA-β-Gal positive cells in A375, <t>MRC5</t> and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.
    Human Normal Embryonic Lung Fibroblast Cell Line Mrc5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5621 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human normal embryonic lung fibroblast cell line mrc5/product/ATCC
    Average 99 stars, based on 5621 article reviews
    human normal embryonic lung fibroblast cell line mrc5 - by Bioz Stars, 2026-03
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    1) Product Images from "Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation"

    Article Title: Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0155725

    (A) Quantification of mean number of SA-β-Gal positive cells in A375, MRC5 and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.
    Figure Legend Snippet: (A) Quantification of mean number of SA-β-Gal positive cells in A375, MRC5 and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.

    Techniques Used: Irradiation, Labeling, Expressing, Western Blot, Activation Assay, Positive Control, Control, Fluorescence, Staining



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    (A) Quantification of mean number of SA-β-Gal positive cells in A375, <t>MRC5</t> and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.
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    (A) Quantification of mean number of SA-β-Gal positive cells in A375, <t>MRC5</t> and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.
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    (A) Quantification of mean number of SA-β-Gal positive cells in A375, <t>MRC5</t> and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.
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    (A) Quantification of mean number of SA-β-Gal positive cells in A375, <t>MRC5</t> and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.
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    Image Search Results


    (A) Quantification of mean number of SA-β-Gal positive cells in A375, MRC5 and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.

    Journal: PLoS ONE

    Article Title: Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation

    doi: 10.1371/journal.pone.0155725

    Figure Lengend Snippet: (A) Quantification of mean number of SA-β-Gal positive cells in A375, MRC5 and 92–1 cells at various time points post X-ray radiation (10 Gy). (B) Quantification of mean number of SA-β-Gal positive cells in 92–1 cells at various time points post-irradiation. (C) Quantification of mean number of Ki67 positive cells in 92–1 cells at indicated time points post-irradiation. (D) Quantification of mean number of EdU labeled cells in 92–1 cells at indicated time points post X-ray radiation (10 Gy). (E) Up: The protein levels of p53 and p21 expression measured by western blotting, indicating persistent p53/p21 pathway activation after ionizing radiation treatment. OCM-1 was a positive control for p53. Down: The protein levels of p16 INK4a , pRB and RB expression measured by western blotting. Data are mean ± s.e.m. (n = 3). Ctrl: non-irradiated samples. β-actin: loading control. (F) Representative micrographs of F-actin fluorescence stained 92–1 cells. Scale bar: 20μm. F-actin was stained with phalloidine-FITC.

    Article Snippet: Human malignant melanoma A375 cells (A375) and human normal embryonic lung fibroblast cell line MRC5 [ ] were purchased from the American Type Culture Collection.

    Techniques: Irradiation, Labeling, Expressing, Western Blot, Activation Assay, Positive Control, Control, Fluorescence, Staining